ENDODEOXYRIBONUCLEASE ECORI Meaning and
Definition
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Endodeoxyribonuclease ECoRI is a type II restriction enzyme that is commonly found in the bacterium Escherichia coli (E. coli). It is widely used in molecular biology and genetic engineering applications due to its ability to recognize specific DNA sequences and cleave the DNA at these sequences.
ECoRI belongs to the endodeoxyribonuclease (endonuclease) category, which means it cleaves the DNA chain internally, rather than at the ends. This enzyme recognizes and specifically binds to a specific DNA sequence known as the ECoRI recognition site, which consists of six base pairs: 5'-G-A-A-T-T-C-3'. Once bound to its recognition site, ECoRI cleaves the DNA backbone within this sequence, resulting in two fragments with sticky ends.
The term "endodeoxyribonuclease" refers to an enzyme that cleaves phosphodiester bonds within the DNA chain. ECoRI cleaves the DNA backbone between the G and A nucleotides within its recognition site, resulting in fragments with protruding 5'-overhangs that can easily hybridize with complementary sticky ends.
ECoRI is widely used in recombinant DNA technology, as its precise cleavage activity allows for the insertion of foreign DNA into a plasmid vector. This enzyme has been extensively studied and has made significant contributions to the understanding of DNA structure, function, and manipulation.
Overall, Endodeoxyribonuclease ECoRI plays a critical role in molecular biology and genetic engineering, providing researchers with a powerful tool for manipulating and studying DNA.
Common Misspellings for ENDODEOXYRIBONUCLEASE ECORI
- wndodeoxyribonuclease ecori
- sndodeoxyribonuclease ecori
- dndodeoxyribonuclease ecori
- rndodeoxyribonuclease ecori
- 4ndodeoxyribonuclease ecori
- 3ndodeoxyribonuclease ecori
- ebdodeoxyribonuclease ecori
- emdodeoxyribonuclease ecori
- ejdodeoxyribonuclease ecori
- ehdodeoxyribonuclease ecori
- ensodeoxyribonuclease ecori
- enxodeoxyribonuclease ecori
- encodeoxyribonuclease ecori
- enfodeoxyribonuclease ecori
- enrodeoxyribonuclease ecori
- eneodeoxyribonuclease ecori
- endideoxyribonuclease ecori
- endkdeoxyribonuclease ecori
- endldeoxyribonuclease ecori
- endpdeoxyribonuclease ecori
Etymology of ENDODEOXYRIBONUCLEASE ECORI
The word "Endodeoxyribonuclease ECoRI" is a combination of different elements that are derived from various sources:
1. Endodeoxyribonuclease:
- "Endo-" is a prefix derived from Greek, meaning "within" or "inside".
- "Deoxyribonuclease" is a word composed of "deoxyribonucleic acid" (DNA) and "-ase" (suffix indicating an enzyme). It refers to an enzyme that breaks down DNA by catalyzing the hydrolysis of phosphodiester bonds.
2. ECoRI:
- "Eco" is taken from the first letters of Escherichia coli, a bacterium used extensively in biological research.
- "R" is derived from the strain of E. coli (RY13) from which the enzyme was first isolated.
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