PRIMED IN SITU LABELING Meaning and
Definition
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Primed in situ labeling (PRINS) is a molecular biology technique used for the specific and localized detection and labeling of DNA sequences on chromosomes or other DNA samples. It involves the synthesis of complementary DNA strands using a primer that is specific for the target DNA sequence to be detected or labeled.
In PRINS, the DNA sample is subjected to a series of denaturation and annealing steps. During denaturation, the double-stranded DNA sample is heated to separate its strands. Then, a DNA primer that is complementary to the target DNA sequence is added. This primer anneals to the target sequence during the subsequent annealing step.
The DNA polymerase enzyme is then used to extend the primer and synthesize a complementary DNA strand. This synthesis process incorporates labeled nucleotides, such as fluorescently labeled or biotinylated nucleotides, into the growing DNA strand. As a result, the target DNA sequence becomes labeled with the fluorescent or biotin tag.
PRINS allows for the detection and visualization of specific DNA sequences within cells or tissues, providing valuable information regarding genetic variations, chromosomal abnormalities, or gene expression patterns. It is often used in cytogenetic research, diagnostic applications, and genome mapping studies. The technique offers advantages such as high sensitivity, specificity, and spatial resolution, making it a powerful tool in molecular biology and genetics research.
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