SITE SPECIFIC MUTAGENESIS Meaning and
Definition
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Site-specific mutagenesis is a molecular biology technique that involves the deliberate introduction of specific mutations into a DNA sequence of interest. It allows researchers to study the functional consequences of these mutations and their effects on protein structure and function. This technique is widely used in various areas of biological research, including genetic engineering, drug discovery, and understanding the molecular basis of diseases.
The process of site-specific mutagenesis typically involves the use of mutagenic primers or oligonucleotides that are designed to introduce specific changes in the target DNA sequence. These primers contain the desired mutation(s) and are synthesized to be complementary to the DNA template. The primers are then used in a polymerase chain reaction (PCR) or another DNA amplification method to amplify the mutated DNA fragment.
The amplified DNA can then be inserted into an appropriate expression system, such as a plasmid or viral vector, and introduced into a host cell. The mutated DNA is then transcribed and translated, resulting in the production of mutant proteins. These mutant proteins can be analyzed and compared to the wild-type (non-mutated) proteins to determine the effects of the introduced mutations.
Site-specific mutagenesis is a valuable tool in molecular biology research as it helps to elucidate the functional importance of specific amino acids in proteins, identify critical regions for protein-protein interactions, and study the effects of specific mutations on protein activity, stability, and specificity. It has proven instrumental in advancing our understanding of biological processes and has significant implications in fields like biotechnology, medicine, and agriculture.
Common Misspellings for SITE SPECIFIC MUTAGENESIS
- aite specific mutagenesis
- zite specific mutagenesis
- xite specific mutagenesis
- dite specific mutagenesis
- eite specific mutagenesis
- wite specific mutagenesis
- sute specific mutagenesis
- sjte specific mutagenesis
- skte specific mutagenesis
- sote specific mutagenesis
- s9te specific mutagenesis
- s8te specific mutagenesis
- sire specific mutagenesis
- sife specific mutagenesis
- sige specific mutagenesis
- siye specific mutagenesis
- si6e specific mutagenesis
- si5e specific mutagenesis
- sitw specific mutagenesis
- sits specific mutagenesis
Similar spelling word for SITE SPECIFIC MUTAGENESIS
- Site Specific Mutageneses.
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